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KMID : 0613820100200030374
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Journal of Life Science
2010 Volume.20 No. 3 p.374 ~ p.380
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Purification and Identification of Inhibitory Compounds on Helicobacter pylori from Cheongmoknosang Callus for Biomass
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Yun Dong-Hyuck
Cha Woen-Seup
Lee Seon-Ho
An Bong-Jeun
Kim Jeung-Hoan
Chun Sung-Sook
Bae Jong-Ho
Cho Young-Je
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Abstract
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The objective of this research was to evaluate the inhibitory activities of phenolic compounds isolated from Cheomoknosang callus on Helicobacter pylori. Total phenolic compounds of 80% ethanol extracts from callus were 15.3 ㎎/g. The activity of H. pylori inhibition at 80% ethanol extracts from Cheongmoknosang callus was determined as 14 ㎜ clear zone. Isolation of inhibitory compounds was carried out on Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient elution procedure of increasing MeOH in H₂O. The chemical structure of the inhibitory compound against Helicobacter pylori was confirmed as protocatechuic acid, chlorogenic acid, caffeic acid and rosemarinic acid by spectroscopic analysis of FAB-MS, NMR and IR spectrum.
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KEYWORD
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Cheongmoknosang callus, Helicobacter pylori, purification, identification
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